Holographic matrix, tertyshny, Georgy Georgievich

Physicist tertyshny, Georgy Georgievich – Cand. tech. Sciences.
In 2000 -2001 G. G. Club “Dia-Elite” of the Russian Diabetic Association (RDA) organized the President’s plan RDA M. Bogomolov, performing the first successful experiments on distant tissue transplantation of a healthy pancreas in rats with experimental diabetes mellitus. The group of rats called diabetes. In another place he revealed a healthy newborn rats were removed, the pancreatic tissue. Then, using special laser and broadband radipole formed in the device called the localizer photons, information from healthy tissues transferred on the sick rats. Patients rats was observed a significant decrease of blood sugar levels and increased survival compared to the control group. Then these experiments have been successfully replicated in many countries around the world.

Patents №2005104490
Device and method multiracial laser spectroscopy
Document type:application
Authors: tertyshny, Georgy Georgievich No. RU) Chuchalin, Alexander Grigorievich No. EN), Maevsky Evgeny Il’ich №RU), Teacher Michael L. No. EN), Khan To # EN)
The patent owner/applicant: Applicant(s): tertyshny, Georgy Georgievich No. RU) Chuchalin, Alexander Grigorievich No. EN), Maevsky Evgeny Il’ich №RU), Teacher Michael L. No. EN), Khan To # EN)
IPC: G01N21/63
Publication date: 10.08.2006

NONINVASIVE TRANSFER OF A FREQUENCY-AMPLITUDE COMPOSITION OF THE ELECTROMAGNETIC FIELD OF BACTERIA WITH THE PURPOSE OF THEIR CORRECTION OF THEIR FUNCTIONING

V.L. Eventov1, G.G. Tertyshny2, M.Yu. Andrianova1
1 Russian Scientific Center for Surgery. Academician B.V. Petrovsky RAMS,
2 Institute of Control Sciences, Russian Academy of Sciences. V.A. Trapeznikova

NONINVASIVE TRANSFER OF PULSE-AMPLITUDE COMPONENT OF BACTERIA ELECTROMAGNETIC FIELD FOR CORRECTION OF THEIR FUNCTIONING

B.L. Eventov, G.G. Tertyshnyy, M.Yu. Andrianova

Bacteria, in addition to the material cell structure, have an individual field structure. This structure forms and determines the cycle of bacterial functioning. It was shown that 41-43% of vancomycin-resistant strains of bacteria Enterococcus hirae ATCC 51575 acquired sensitivity to vancomycin and killing them after laser irradiation field structure vancomycin-sensitive strain of bacteria Enterococcus hirae ATCC 10541. At the same time, laser irradiation vancomycin-resistant bacterial strain Enterococcus hirae ATCC 51575 without a donor did not lead to their death, as well as in the control group.

Besides, there is an individual field structure. This structure forms and determines a cycle of bacteria functioning. It was shown that 41-43% steady to vancomicin bacteria (strain Enterococcus hirae ATCC 51575) had got sensitivity to vancomicin and died after laser irradiation with field structure of sensitive to vancomicin bacteria (strain Enterococcus hirae ATCC 10541). At the same time laser irradiation of steady to vancomicin bacteria (strain Enterococcus hirae ATCC 51575) without donor had not led to their destruction, also as well as in control group.

Keywords: genetic information, noninvasive transfer, bacteria, laser.

INTRODUCTION

Bacteria, in addition to material cellular structure, have individual field structure [1, 2, 3, 4]. This structure creates and defines a cycle of functioning of bacteria. At exogenous field influence change of functioning of bacteria is possible. For effective impact on a specific species of bacteria the electromagnetic field of external influence has to be analogous in the majority of parameters to an internal field of bacteria the Research objective: using the laser scanner, to estimate transfer of sensitivity to Vancomycinum from a strain of Enterococcus hirae (ATCC 10541) sensitive to Vancomycinum, on the strain of Enterococcus hirae (ATCC 51575) steady against Vancomycinum; to confirm that the revealed changes in a strain, steady against Vancomycinum, demand presence at laser equipment of the donor (a strain of Enterococcus hirae ATCC 10541 sensitive to Vancomycinum).

MATERIALS AND METHODS
For reading and transfer of field structure of bacteria used two-mode the helio-neon laser TE-3 scanner. A laser beam, passing through an electromagnetic field of bacteria, it is modulated by its field structure. This modulation is expressed in change of frequency amplitude characteristic of a laser beam. The modulated by a frequency and amplitude component of the field of bacteria, laser beam gets on similar bacteria where it is detected, partially altering
their field structure and functioning. As the recipient used Enterococcus hirae ATCC 51575 (a strain, steady against Vancomycinum), as the donor – Enterococcus hirae ATCC 10541 (a strain, sensitive to Vancomycinum).

PREPARATION OF CULTURES OF MICROORGANISMS

Previously both strains of bacteria tested for confirmation of their resistance / sensitivity to Vancomycinum. For this purpose cultures of cells applied with strips on Petri dishes with a medium (agar) and placed on an agar surface disks with Vancomycinum (5 mkg). Then Petri dishes placed in the thermostat for 24-48 hours at a temperature of 30-35 °C and analyzed zones of suppression of growth of microorganisms. On Petri dishes with a sensitive strain the pure zone of suppression of growth was formed (~ 3,8 mm from a disk), on Petri dishes with a resistant strain the muddy zone of suppression of growth was formed (~ 0,8 mm from a disk) that confirmed a phenotype of microorganisms.

EXPERIMENT

The strain of Enterococcus hirae ATCC 10541, sensitive to Vancomycinum, was besieged centrifuging at 1500 rpm, then deleted a supernatant, placed a deposit on glass integumentary glass and covered from above with the second glass integumentary glass. The package from two glasses of a deposit of a strain of Enterococcus hirae ATCC 10541, sensitive to Vancomycinum, was used as the donor in laser equipment. The recipient (a strain of Enterococcus hirae ATSS, steady against Vancomycinum) 51575 applied on 6 Petri dishes with an agar on 1 drop with a diameter from 2 to 3 mm. In 2 Petri dishes (1 and 1a) the recipient processed the laser beam which passed through integumentary glasses with the donor (fig. 1); in 2 Petri dishes (2 and 2a) the recipient processed the laser beam which passed through empty integumentary glasses. 2 more Petri dishes with culture of the recipient (3 and 3a) isolated in the boxing shielded by metal in the certain room and used as the control which was not affected by the laser. Right after experiment from each Petri dish selected portions of the processed cells of culture of the recipient which placed in bottles from 8 ml of a medium (broth), and tests incubated in the thermostat night at a temperature of 30-35 °C. Each bottle with broth was marked the corresponding code of a cup.

RESULTS

In control samples (2, 2a, 3 and 3a) average concentration of cells in 4 hours made 9,38. 108 cells/ml whereas in the samples subjected to influence of the modulated Enterococcus hirae ATCC 10541 (1, 1a) (a strain, sensitive to Vancomycinum) a laser beam average concentration of cells made 4,127. 108 cells/ml. The difference of concentration of cells made 44% that convincingly proves influence of the postponed properties of a strain of bacteria, sensitive to Vancomycinum, on insensitive.

TURBIDIMETRIC ANALYSIS

For the purpose of verification of the obtained data, the method of agar cups was adapted for determination of compliance of density of cells of optical density at 530 nanometers. Cultures of cells at which spectral absorptive power made 0,075/0,092(1 and 1a) and from 0,382 to 0,420 (2, 2a, 3, 3a) parted with broth to degree 10-6. On 1 ml of each cultivation mixed with a tripsinovy soy agar, dried up, 48-72 hours at 30-35 °C incubated and read out quantity of colonies. Solution Vancomycinum-HCl prepared from a standard Vancomycinum-HCl, United States Pharmacopeia (USP) containing 100 mkg / a bottle, parting it 10 ml of sterile treated water that gave final concentration of 10,050 mkg/ml. This solution was dissolved with sterile treated water to concentration of 1 mkg/ml in day of research. Using the spectrophotometer, each culture in broth was standardized on density of cells. In pilot and control samples in the test tubes containing 9 ml of broth and 1 ml of solution Vancomycinum – HCl (10 mkg/ml), brought 0,25 ml of the corresponding standardized cultures of microorganisms of the recipient and incubated in a water bath 4 hours at 37 °C. Results are provided to tab. 1. Thus, it was confirmed that 43% (r <0,001) bacteria of a strain of Enterococcus hirae ATCC 51575, steady against Vancomycinum (1 and 1a) gained sensitivity to Vancomycinum and died after a laser exposure their field structure of a strain of bacteria of Enterococcus hirae ATCC 10541, sensitive to Vancomycinum. At the same time, the laser exposure of bacteria of a strain of Enterococcus hirae ATCC 51575, steady against Vancomycinum (2 and 2a) without donor did not lead to their death, as well as in control research (3 and 3a).

CONCLUSIONS
1. 10541 frequency and amplitude components of their field structure scanned by the laser the TE-3 scanner from a strain of bacteria of Enterococcus hirae ATSS, sensitive to Vancomycinum, led of 43% of bacteria of a strain of Enterococcus hirae ATCC 51575, steady against Vancomycinum, to death.

2. The laser exposure without donor of bacteria of a strain of Enterococcus hirae ATCC 51575, steady against Vancomycinum, did not lead to their death.

 


Fig. 1. Scheme of the laser scanner. 1 – laser, 2 – laser beam, 3 – cover glasses, 4 – donor – vancomycin-sensitive strain of bacteria Enterococcus hirae ATCC 10541. 5 – laser beam modulated by the frequency-amplitude component of the bacterium field of Enterococcus hirae ATCC 10541 sensitive to vancomycin, 6 – the recipient is stable
To vancomycin strain of bacteria Enterococcus hirae ATCC 51575.7 – agar, 8 – petri dish

 

Table 1. Correspondence of optical density to the number of bacteria colonies

MANAGEMENT OF NORMALIZATION OF THE FUNCTIONING OF THE CELLS OF THE ORGANISM BY IRRADIATION BY RADIO WAVES MODULATED WITH INFORMATION ON THE FUNCTIONING OF SIMILAR HEALTHY YOUNG CELLS
Ostrovsky YI.1, Tertyshny G.G.2 (Institute of Control Sciences, Russian Academy of Sciences, Moscow)
EVVENTV VL.3 (Russian Research Center for Surgery named after Academician Petrovsky, Moscow)

The results of experiments on the treatment of diabetes mellitus in rats by their irradiation with radio waves modulated by electromagnetic radiation of biostructures by means of a laser are presented. Considered sources of electromagnetic radiation of biostructures are high-frequency mitogenetic radiation and low-frequency radiation caused by changes in the permeability of cell membranes. It is shown that mitogenetic radiation can not provide the resulting therapeutic effect. Hypotheses are proposed about the source of radiation, which provides a therapeutic effect, and on the mechanism of modulation of radio waves by radiation of biostructures. Key words: treatment of diabetes mellitus, mitogenetic radiation, permeability of cell membranes, modulation of radio waves, laser.
1. Introduction
In [2] and [8], treatment of alloxan diabetes mellitus in rats with the help of broadband electromagnetic radiation (SHEI) acting on the pancreas in the range of medium radio waves modulated by electromagnetic radiation of biostructures is described. Two sources of electromagnetic radiation of biostructures are known: · high-frequency radiation of chromosomes in the ultraviolet region of the light spectrum (the so-called mitogenetic radiation); Low-frequency electromagnetic radiation with frequencies less than 1000 cps, which is due to changes in the permeability of cell membranes. There are hypotheses about a radiation source that provides a therapeutic effect when acting on the pancreas of the SHI, electromagnetic-modulated biostructures, and the mechanism of modulation of radio waves by this radiation.

 

2. Mitogenic radiation

The effect of mitogenic radiation is in detail described in work [3]. Mitogenic radiation is the ultraviolet light arising at exothermic chemical reactions. This radiation was revealed A.G. Gurvich prieksperiment with onions backs. Actively sharing onions back tip cells at distance of 2 – 3 mm initiated a mitosis (cell fission) in tissue of another, the onions back which are chemically isolated from it. Follows from A.G. Gurvich’s experiments, h that information signal is transmitted by means of the low-strong radiation having weak absorption in quartz, strong in glass, good reflection from a specular metallic surface. The range of this radiation which is in the range of lengths of waves 1800 – 2500 angstrom (ultra-violet area of a light range) manages to be received by means of a quartz prism and the biological radiation detector (see below). This range is different for different types of organisms and for different types of cells of a specific organism. A source of mitogentichesky radiation in these experiments was DNA of chromosomes of a kernel of a cell, however mitogenic radiation is observed also in different enzymatic reactions out of a live organism. So, for example, this radiation arises at addition to solution of nucleic acid of enzyme of a phosphobasin. Absolutely identical radiation spectrum arises also when splitting lecithin. Two types of nucleic acids are known: deoxyribonucleic acid (DNA) which contains genetic information in chromosomes, and ribonucleic acid (RNA) which participates in synthesis of proteins. Mitogenic radiation of living cell is caused by chemical reactions of DNA. Mitogenic radiation is observed also at reactions between some non-organic substances. Intensity of mitogenic radiation it is insignificant it is small (about several thousand photons a second on 1 sq. cm) therefore detection and spectrum analysis of this radiation is accompanied by considerable difficulties. The technique of detection of an ultraviolet by physical methods at its sufficient intensity is quite simple, however for mitogenic radiation because of its insignificant intensity this technique cannot be used. Photographic plates even of the highest sensitivity are unsuitable. There is only a method of the counter of photons which, however, is suitable only for the strongest and long sources of mitogenic radiation. For researches of the biological sources of radiation demanding fast work (short-term radiation burst, a spectral analysis, etc.) the only methods of detection of mitogenic radiation are biological detectors. As the biological detector the culture of yeast on an agar is used generally. At radiation by mitogenic radiation intensity of cell fission of yeast which is found by means of a microscope by the visual calculation demanding considerable skill of the operator increases. Living cell can with a certain probability begin to share also without any external influence, however radiation by mitogentichesky radiation sharply increases probability of its division. At the same time the irradiated cell for some time itself becomes a source of secondary radiation which influences the next cells. Short-term secondary radiation arises also at other external impacts on a cell (shock chilling, centrifuging, a transmission of small direct or variable electric current, radiation by a laser beam). A.V. Budagovsky offered a hypothesis that the radiation of DNA of chromosomes is the hologram which contains information on an organism in general. This hypothesis was confirmed with experiment with tyloses of fruit plants [1]. A tylosis – a fabric new growth at plants on a wound surface. Cells of a tylosis are similar to stem cells of an animal organism – they can be differentiated in any cell of a plant. The tylosis looks the translucent mass of irregularly sharing cells which, in the absence of control signals, seldom pass to a differentiation. In the course of experiment more than 1000 identical tyloses received from klonalno the multiplied fabrics of cherry and cultivated in strictly identical conditions were separated into 4 groups. In the first (control) group tyloses were not exposed to any influences. In the second group tyloses were irradiated with a helium-neon laser beam. In the third group tyloses were also irradiated with a laser beam, but before a tylosis the inductor – escape of cherry of the same grade was installed. In the fourth group radiation of a tylosis was made according to the scheme of record of the volume hologram offered by Yu.N. Denisyuk [4]. At the same time the tylosis was placed between the laser and a plant – the inductor. The laser beam dissipated on an inductor surface. Between the laser and the inductor there was a zone of standing waves which are formed as a result of an interference of the reference light wave radiated by the laser with light waves which are reflected by the inductor in the direction of the laser. In this zone there was a translucent tylosis. The waves reflected from the inductor bear information on structure of the inductor in a phase form. As a result of an interference of these waves with reference wave phase information turns in amplitude which can be recorded by the photosensitive environment. In this case such environment is tylosis fabric. The part of cells of a tylosis appears in puchnost of standing waves. According to A.V. Budagovsky’s hypothesis, in these cells the centers of a new growth of the differentiated fabrics are created. The result of experiment was the following: the number of escapes of cherry in control group made 3,6 ± 2%, in the second and third groups – 8,3 ± 5,6%, and in the fourth group (holographic information transfer) – 26,1 ± 9,2%. Thus, experiment confirmed a hypothesis of holographic structure of radiation of DNA.

3. The low-frequency electromagnetic radiation caused by changes of permeability of cellular membranes

Low-frequency electromagnetic radiation of living cells is in details described in works [5], [7]. Many living cells are peculiar electrochemical generators. These generators actually represent flows of the ions moving under the influence of chemical concentration gradients. They create low-frequency (with frequencies from hertz shares to 1 kHz) the electromagnetic field reflecting functioning of appropriate authorities of an organism. The electric component of this field in the form of records of the electric potential changing in time widely is used in electrophysiologic issledovany and in diagnosty diseases of the person (an eletrokardiografiya, an electroencephalography i.t.p.) . Studying of a magnetic component of low-frequency electromagnetic radiation of living cells requires the special highly sensitive equipment – a squid magnetometers – and high-quality shielding of the room from external electromagnetic fields. For ensuring high sensitivity a squid magnetometers sensing devices of these devices are cooled with liquid helium for the purpose of use of effect of superconductivity. The internal space of each cell filled with intracellular substance is separated from the extracellular liquid surrounding a cell by a membrane which has different permeability for ions of the different types which are present in and out of a cell. Ion concentrations in intracellular substance and in extracellular liquid strongly differ. Especially it belongs to ions of sodium, potassium, chlorine and organic anions. For example, in steady-state conditions in muscles and nerves of mammals ion concentration of potassium in a cell is tens times higher, than outside, and ion concentration of sodium and chlorine, on the contrary, significantly higher outside. Under the influence of concentration gradients ions move through a membrane, overcoming its resistance. These flows of ions arising under the influence of not electric forces form electric currents which are primary bioelectric generator generating both a bioelectric, and biomagnetic field. The cellular membrane possesses most high-permeability for potassium ions. When they begin to follow from a cell, between internal and outside surfaces of a membrane the potential difference interfering their effluence is formed. At a certain value of this transmembrane potential the effluence of potassium ions from a cell stops. The corresponding transmembrane potential of rest usually matters about 90 mV. Cellular membranes change the permeability for different ions under the influence of the different stimulating influences (electric current, chemical influence, electromagnetic radiation, etc.) . If the size of the stimulating influence exceeds some threshold value, there is a jump of membrane permeability and there is an excitation impulse – fast changing of transmembrane potential then throughout certain time as a result of complex oscillatory processes of change of ionic pronitsayemost rest potential is recovered. These oscillatory processes are strictly individual for different organisms and for different types of cells of a specific organism. They depend also on a condition of a cell. Pulse change of transmembrane potential is called action potential. Amplitude of action potential can reach several tens millivolts. Action potential is followed by fluctuations of amplitude of membrane current and generation of the corresponding electromagnetic field. Power of the electromagnetic radiation caused by izmneniye of permeability of cellular membranes is incomparable is insignificant the low power of mitogenic radiation above. If between intracellular areas of the next cells there are contacts with small resistance, then through these contacts the stimulating action of membrane current extends to the next cells, leading to coverage process by excitation of all fabric. Such mechanism of transfer of excitation operates in a myocardium. At synoptic type of connection between cells (for example, in nervous tissue) in a synapse zone the excited cell influences a membrane of the next cell chemicals (mediators), causing development in this cell of action potential by selective to change – a niya of permeability of its membrane for different ions. Can affect the next cells and generated it is excited – ache a cell an electromagnetic field. In fabric of sense organs receptor cells can be excited and generate poten-
tsiat actions under the influence of pressure, light and other incentives.

4. Treatment of an alloksanovy diabetes mellitus at rats by means of broadband electromagnetic radiation

In YIP RAHN G.G. Tertyshny developed the device for direct transfer of mitogenic radiation of living cell from the donor to the recipient by means of a laser beam. This device consists of the 2 MW helium-neon laser with automatic thermofrequency fixing of radiation and the optical block. The laser has the radiation modes two combined, orthogonally polarized. The system of thermofrequency fixing of laser emission provides equalizing of radiant powers in these modes. The optical block consists of two translucent mirrors, the translucent biological object located between these mirrors – the donor and the justified device. Behind the optical block the biological object – the recipient is located. The donor and the recipient are located on an optical axis of a laser beam. Having passed through object – the donor, the laser beam is repeatedly reflected by translucent mirrors of the optical block, forming system of standing waves between these mirrors. In zones of puchnost of these waves there is a modulation of a laser beam the electromagnetic radiation of cells of biological object – the donor. Part of the laser beam modulated thus, passing through a translucent mirror, irradiates biological object – the recipient. By means of the justified device partial retroreflection of a beam in the laser cavity is provided. In the course of researches on plants it became clear that if between the optical block and the recipient to establish an opaque valve, then the recipient continues to be irradiated with the radiation of the donor. This results from the fact that along with biological information which is transferred directly by a laser beam the laser generates the broadband electromagnetic radiation (BER) in the frequency range of 50 kHz – 2 MHz (range of medium radio waves). It turned out that these radio waves are modulated by biological information which affects live organisms too. Experiments on use of this information for treatment of an alloksanovy diabetes mellitus at rats [2], [8] were made. The diabetes mellitus was caused by an injection of solution of an alloksan with concentration of 200 mg/kg of weight of an animal. All rats from control group died for the 4th day after this injection because of development of a diabetes mellitus. Uroven of glucose in blood of these rats exceeded 30 mmol/l. Rats of experimental group with the day before yesterday after an injection of an alloksan were irradiated with ShEI received as a result of passing of a laser beam through svezhevydelenny drugs of a pancreas and spleen of a newborn rat. 80% of animals from this group after influence of ShEI had a reliable decrease in level of glucose in blood. These rats recovered. 20% of rats died against the expressed hyperglycemia on the 6th and 7-esutka after an injection of an alloksan.
At histologic research of a pancreas of the dead of animals of control group reduction of number and the sizes of islands of Langermans, falloff of quantity of the b-cells producing insulin in these islands is revealed. At the same research of a pancreas of rats of experimental group who were subjected to euthanasia in 1,5 months after an injection of an alloksan activation of regeneration processes in a pancreas is found. Glucose level in blood at these animals made less than 10 mmol/l.

5. Researches of influence of the radio waves modulated by the radiation of healthy cells of a pancreas, on patients with a diabetes mellitus of rats, the surgeries of the Russian Academy of Medical Science which are carried out in the Russian scientific center

In the Russian scientific center of surgery of the Russian Academy of Medical Science researches of influence on patients with a diabetes mellitus of adult rats of the radio waves modulated by information on normally functioning cells of a pancreas of 10 – 14 day infant rats were conducted. The diabetes mellitus model at rats was created by intraperitoneal administration of solution of an alloksan. Originally to six 3-month-old males of rats of population of VISTAR, weighing from 285 to 310 g, solution of an alloksan was intraperitoneally entered by concentration of 200 mg/kg of weight. At all animals for the second day concentration of glucose in blood increased and on 3 – the 4th days all experimental rats died against incredible concentration of glucose in blood. Then other party from ten males of rats of population of VISTAR solution of an alloksan with the same concentration was also entered and in 3 hours their radiation was begun with the radio waves modulated by information on normally functioning cells of a pancreas and spleen of 10 – 14 day infant rats. For this purpose the pancreas and a spleen were withdrawn from previously lulled infant rats. Drugs of these bodies was located on microscope slide (fig. 1).

Fig. 1. Pancreas drug preparation

Microscope slide with drug was located in the optical block located in front of the helium-neon laser with automatic thermofrequency fixing of the radiation (fig. 2). Drug was on an optical axis of a laser beam. Longevity of the made drug does not exceed 8 – 10 minutes therefore each drug was used for radiation for only 5 minutes. In the course of each session of radiation were used gland and a spleen of four infant rats. At the same time the general radiation time of rats with use of drug of each of these bodies throughout a session of radiation made 5*4 = 20 minutes. In total 2 sessions of radiation were held. As a result of radiation concentration of glucose in blood of all rats was stabilized in the range of 6 – 7,5 mmol/l, any rat did not die. In 7 days all rats were destroyed for studying of internals. Results of research are presented on fig. 3.
Thus, the effect of scanning by the helium-neon laser of information on normally functioning cells of a pancreas and spleen of 10 – 14 day infant rats and transfers of this information a sick diabetes mellitus was visually shown to adult rats by means of the radio waves modulated by this information. It should be noted that in the majority of the Russian vivariums purity of the VISTAR line is lost therefore instead of the term “VISTAR line” the term “VISTAR population” is used. At the same time essential dispersion of the parameters characterizing a condition of rats therefore confirmation of the gained effect by carrying out a series of similar experiments, and also studying and exarticulation of a useful signal is planned further is observed. Independently there is a problem of record and reproduction of a useful signal, a soderzhashchegoinformation about normal functioning of cells of a pancreas and spleen, for the purpose of repeated use of this signal.



6. The electromagnetic radiation of biostructures providing modulation of radio waves, and a role of strata in this modulation (hypothesis)

In the analysis of the stated above results of pilot studies there are following questions. · What source of electromagnetic radiation of biostructures provides modulation of the radio waves generated by the laser – the high-frequency mitogenic radiation of DNA of chromosomes or low-frequency electromagnetic radiation caused by changes of permeability of cellular membranes? · As there is a modulation of a laser beam radiation bio –
structures? · How there is a generation of radio waves the laser? High frequency of mitogenic radiation (ultra-violet area of a light range) cannot get through fabrics of a live organism to a pancreas. Besides, it is insignificant the low power of the mitogenic radiation found only by biological detectors and counters of photons, does almost improbable a possibility of modulation by this radiation of a laser beam. Frequency of the electromagnetic radiation caused by changes of permeability of cellular membranes does not exceed 1000 Hz. The radio waves modulated by such radiation with a carrier frequency in the range of 50 kHz – 2 MHz freely get through fabrics of a live organism. Power of this radiation it is incomparable higher than the power of mitogenic radiation, its electric component effectively is registered the existing standard medical equipment. Thus, a source of electromagnetic radiation of biostructures which provides modulation of the radio waves generated by the laser, can be only the low-frequency electromagnetic radiation caused by changes of permeability of cellular membranes. Under certain conditions in a laser tube spontaneously there are plasma self-oscillations which received the name of strata [6]. Usually presence of strata at a laser beam is considered as the undesirable phenomenon, and try to get rid of them, but it works well not always. Frequency of strata happens different. Frequency of 50 kHz – 2 MHz can be frequencies of strata. There is no generator with such frequency in the scheme of the helium-neon laser used by us. If in plasma of the laser there are strata, then they are present at its beam which passes through pancreas drug. It is possible to assume that at the same time there is amplitude modulation of strata the low-frequency electromagnetic radiation of b-cells of this gland which is caused by changes of permeability of cellular membranes. The modulated beam is partially reflected back in the laser cavity. It is known that self-oscillations of plasma are a source of electromagnetic radiation with the same frequency [9]. At the same time the tube of the laser can be considered as the vibrator radiating radio waves with the carrier frequency equal to the frequency of strata which is modulated by biological information. Check of the offered hypotheses of frequencies of electromagnetic radiation of cells of a pancreas which provides treatment of an alloksanovy diabetes mellitus at rats about the mechanism of generation of radio waves by the laser and about the mechanism of modulation of these radio waves the radiation of cells requires a pilot study.

Literature
1. BUDAGOVSKIY A.V. Remote intercellular interaction. – M.: NPLTs “EQUIPMENT”, 2004. – 103 pages.
2. GARYaEV P.P., BREAKING A.A., MUHINA I.V., LEONOVA – GARYAEVA E.A., BREAKING N. G. Influence of the electromagnetic radiation modulated by biostructures on a current of an alloksanovy diabetes mellitus at rats.//Bulletin of experimental biology and medicine. – 2007. – No. 2. – page 155-158.
3. GURVICh A. and L. Mitogenic radiation. Physical and chemical bases and applications in biology and medicine. – M.: “MEDGIZ”, 1945 – 284 pages.

4. DENISYUK YU.N. About display of optical properties of object in wave front of the radiation disseminated by it.//The USSR is GIVEN. – 1962. – t.144. – No. 6. – page 1275 – 1278.
5. KNEPPO of the Item, TITOMIR L.I. Biomagnetic measurements. M.: “Energoatomizdat”, 1989, 287 pages.
6. NEDOSPASOV A.V. Strata.//Achievements of physical sciences. – 1968. – volume 94. – March. – issue 3. – page 439 – 462
7. PLONSI of River, BARR R. of Bioelektrichestvo. Quantitative approach. – M.: “World”, 1992. – 366 pages.
8. TERTYSHNY G.G., GARYaEV P.P. Wave genetic nanotechnologies of management of biosystems. Theory and experiments.//New medical technologies / New medical equipment. – 2007. – No. 7. – page 49-64.
9. STURROCK P.A., BALL R.H., BALDWIN D.E. Radiation at the Plasma Frequenci and its Harmonic from a Turbulent Plasma.//The Phisics of Fluids. – 1965. – vol. 8. – No. 8. – p. 1509 CONTROLLING OF NORMALIZATION OF FUNCTIONING OF THE ORGANISM CELLS BY IRRADIATING THEM BY RADIOWAVES MODULATED BY INFORMATION ABOUT FUNCTIONING OF SIMILAR HEALTHY YOUNG CELLS Yuriy Ostrowski, Trapeznikov Institute of Control Sciences, Russian Academy of Sciences, Moscow, Russia; Senior Researcher, Cand. Sci., Associate Professor. Georgiy Tertishniy, Trapeznikov Institute of Control Sciences, Russian Academy of Sciences, Moscow, Russia; Senior Researcher, Cand. Sci. Victor Eventov, Petrovskii Russian Surgical Center, Chief Researcher of the Hemodialysis Laboratory, Dr. Sci, (Eng.ng.), Member of the Russian Medico-Technical Academy and Russian Academy of Natural Sciences.

Abstract: Results of the experiments on treating diabetes of rats by irradiating them by radiowaves modulated by the electromagnetic radiation of the biological structures with the use of laser were presented. Consideration was given to the existing sources of the electromagnetic radiation of the biological structures such as the highfrequency mitogenetic radiation and low-frequency radiation caused by the variations in permeability of the cell membranes. It was demonstrated that the mitogenetic radiation cannot provide the obtained medicinal effect. Hypotheses were proposed about the source of radiation providing the medicinal effect and the mechanism of radiowave modulation by radiation of the biological structures. Keywords: treatment of diabetes, mitogenetic radiation, permeability of the cell membranes, radiowave modulation, laser.

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From the paper on competition of the doctoral dissertation of Candidate of Technical Sciences G.G. Tertyshny (YIP RAHN).
Memories of the founder of YIP RAHN: I.V. Prangishvili is devoted.

G. G. Tertyshny and K.V. Tyuts.

Names of the project.
a) Short name of the project: “Holographic management in biological systems for the purpose of stimulation of development of the stem cells necessary for recovery regeneration and rejuvenation of a human body”.

1. The unrolled name of the project: “A laser hologram technique of reading, forming and broadcast of managing donor and biogenous information for improvement of a state * “sick and/or old cells” of the recipient”.

It happens due to perception the recipient of this information, storing and long-term endogenous and exogenous reconstruction of holographic structural and dynamic images of cells of the donor by simultaneous stimulation of development of own stem cells, to the subsequent their broadcasting in a zone of sick bodies and tissues of the recipient for their regeneration.

2. a) Justification (short) scientific pilot project.

Due to the progress in development of dynamic polarizing holography and developed by us in the way and the structure of information polarizing and holographic management of a condition of sick cells of the recipient without geometrical and their large-scale distortion. It occurs by means of broadcast of holographic managing information from the donor to the recipient in a distant zone and due to simultaneous additional stimulation of development of stem cells for their use in the course of regeneration. We experimentally checked a possibility of perception the recipient of biogenous related information, as allowed to manage a condition of biosystems, up to complete regeneration and rejuvenescence of bodies and fabrics, in the presence of a possibility of a right choice of cells “related – healthy” * the donor, and also optimum managing exposure in the favorable mode of functioning of the laser and holographic device.

b) Justification of the unrolled scientific pilot project.

The new method of forming and broadcast of information from the donor to the recipient and perceptions is offered them to biogenous information for management of processes of life activity in biosystems. The mathematical model of polarizing and dynamic processes of management of biological objects by means of polarizing and laser holographing is given. In the project theoretical bases of broadcast of polarizing and dynamic information are briefly stated and the way implementing it and the device for implementation of holographic management in biological systems is discussed. Some results of use of holographic information and laser transformation for reading and broadcast of dynamic holographic memory of own healthy cells from the donor are given to sick cells of the recipient, and also simultaneous information and holographic stimulation of development of own stem cells by an organism for their further use in processes of regeneration of bodies and tissues of the person. The essence of a technique of information and holographic stimulation of stem cells consists in allocation of own organism of insignificant quantity of own, healthy stem cells and placement them in our polarizing and holographic installation in quality of an additional donor stimulator.

The term “holography” comes from two Greek words holos – everything, full and … a grafiya – the image, and designates the full volume image of object. At a phase (transparent) structure of golografiruyemy object on all space full and its detailed image turns out. For the first time the method of holographing is offered by D. Gabor in 1948 and significantly added by domestic scientists. The traditional method is based on an interference of coherent radiation of any nature. For example, on a photographic plate along with the “alarm” wave disseminated by object send to an object bypass a “basic” or reference wave from the same light source. The picture arising at an interference of these waves containing the complete information about object is fixed on a photosensitive surface. It is called the hologram. At radiation of the hologram or its site reference wave it is possible to see the volume image of all object. The holography is widely used in physics and different areas of equipment (in particular, for recognition of images and coding of information), in acoustics (for detection of internal defects in responsible metal designs of nuclear power plants), etc. The holography has big perspectives during creation of volume cinema and television [1]. This work (as well as some other [2-15, 24]) is continuation of researches on implementation of a hypothesis of holographic properties of biosystems and about a possibility of holographic management of a condition of cells of these systems. We understand change of internal state and structure of cells as a result of managing light, acoustic or electromagnetic influences as holographic management.

At management of biological systems transfer of holographic information from the donor to the recipient is made. During laser and holographic experimental works on plants in 1997 the phenomenon of holographic broadcast of information from the donor to the recipient was revealed [9-15, 23]. The essence of this phenomenon consists in passing of laser radiation through translucent biological cells of the donor which modulate this radiation own polarizing and phase hologram. For steady and distortionless storing in a beam flow of the read-out information the method of vibration-proof polarizing dynamic transformation and the device (sensor converter) was developed for its implementation. In a physical basis of such converter the principle of excess coding of each amplitude-phase disseminating object point in the form of polarizing rings to similar Newton’s fringes is underlain.

In experiments vibration-proof transfer of incoherent holographic information from the donor to the recipient was made. At rather long and purposeful okolorezonansny exhibiting of the recipient there was a phenomenon of holographic management of a condition of cells of the recipient by means of the broadcast information proceeding from the donor and from a donor stimulator of stem cells.

. As a result the condition of cells of the recipient improved, approaching the normal state characteristic of cells of the young and healthy donor.

It is explained by the following reasons. First, it turned out that the main pool of holographic information is not only in amplitude-frequency and phase shift keyings, but also in polarizing and dynamic modulations of corners of Euler. It can be explained with the fact that after reflection and dispersion of a laser beam from each point of the donor arise light a cone of the passing stray radiation in which the orthogonal circular polarization proceeding from the laser will be transformed to its space and conic distribution. At interaction of stray radiation of light cones with the polarizing reference wave synthesized by a sensor converter (a special polarizing quasilens) there are space distributed polarizing rings reminding Newton’s fringes. If living cells, then they are mobile. However, synthesizable polarizing rings, are practically not mobile from each other and concerning the chosen beginning of coordinates chosen in space in which there is a donor object. It happens because of relative coherence information (basic and alarm) points of the donor among themselves. Variable corners of Euler are caused by the space angular microscopic fluctuations of points of the donor corresponding to a dynamic condition of cells of live biological object. These variable corners represent corners between straight lines, tangent to polarizing rings, and coordinate in which points of the donor are considered.

We developed a way and the device for a solution of the problem of dynamic management of biosystems for the purpose of regeneration of bodies and fabrics. The essence of this way and the device consist in passing of laser or electromagnetic radiation through an integral organism or its fragment, for example, through translucent stem biological cells of the donor which modulate this radiation own polarizing and phase hologram. For steady and distortionless storing in a beam flow of the read-out information the method of vibration-proof polarizing dynamic transformation and the device (sensor converter) was developed for its implementation. In a physical basis of such converter the principle of excess coding of each amplitude-phase disseminating object point, for example, in the form of polarizing rings is underlain (like Newton’s fringes).

In experiments vibration-proof transfer of incoherent holographic information from the donor to the recipient was made. At rather long and purposeful okolorezonansny exhibiting of the recipient there was a phenomenon of holographic management of a condition of cells of the recipient by means of the broadcast information proceeding from the donor. As a result the condition of cells of the recipient improved, approaching the normal state characteristic of cells of the donor.

It is explained by the following reasons. First, it turned out that the main pool of holographic managing information is in polarizing and dynamic modulations of corners of Euler. It can be explained with the fact that after reflection and dispersion of a laser beam from each point of the donor arise light a cone of intensity of the passing stray radiation in which the orthogonal circular polarization proceeding from the laser will be transformed to its space and conic distribution. At interaction of stray radiation of light cones with the polarizing reference wave synthesized by a sensor converter (a special polarizing quasilens) there are space distributed managing polarizing rings the reminding Newton’s fringes. If living cells, then they are mobile. However, synthesizable rings, are practically not mobile from each other and concerning the beginning of coordinates chosen in space in which there is a donor object. It happens because of relative space coherence of points of the donor among themselves. Variable corners of Euler are caused by the microscopic amplitude fluctuations of points of the donor corresponding to a dynamic condition of cells of live biological object. These variable corners represent corners between straight lines, tangent to managing polarizing rings, and coordinate in which points of the donor are considered.

Secondly, it was possible to transfer managing polarizing and holographic information from the donor to a distant zone where the recipient was located. The distant zone, as usual, is understood as the distance considerably exceeding the wavelength of the laser probing signal playing a role of a carrier signal. For implementation of process of holographic management the above-mentioned special polarizing quasilens was also developed and made.

Thirdly, the problem of dynamic stability of managing polarizing holograms was solved that appeared especially important for work with live biological organisms. It means that at any micromovements of internal or external structures of the donor or recipient, and also a laser beam (for example, because of seismic mobility of the base on which the laser is installed) concerning cells of the donor, there is the same system of the polarizing rings directed to cells of the recipient.

Fourthly, at holographic polarizing coding and broadcast of managing volume information it was succeeded to solve a problem of preservation of genetic redundancy. This redundancy is understood here in the sense that it is connected with direct and return Fourier transformation which consists, first, in forming and registration of the polarizing managing rings proceeding from each point of the donor and, secondly, in their return Fourier transformation which symmetrically broadcasts these points on the recipient. Direct Fourier transformation gives system of polarizing rings for each point of cells of the donor, and the return – will transform these rings to points similar to points of the donor, but transferred to a distant zone on the recipient. As a result redundancy is provided that when passing through a quasilens, each cellular structure of the donor is transformed to set of volume polarizing cones of standing light wave of intensity. In case of partial deleting or vibration degradation of above-mentioned managing rings which correspond to some point of the donor the rest of rings is sufficient for the correct forming of the corresponding point of the donor.

In it the main differences and advantages of a way and the structure of holographic management of a condition of cells of biological systems consist. At the expense of the above-stated decisions polarizing and dynamic holographic broadcast of managing information without its geometrical and large-scale distortion was received. It also allowed to solve, unresolved so far, a problem of holographic polarizing management of a condition of bodies and fabrics at examinees of animals.

The way and the device are repeatedly checked by us experimentally, as on plants, bacteria, and for fatally sick animals who are in a coma. Rats like Vistar were cured by us of the induced diabetes mellitus (alaksanovy model).

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